Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | DyP activity for Reactive Blue 5 is inhibited when the H2O2 concentration exceeds 0.2 mM at a fixed DyP concentration of 0.6 nM. At 2.8 nM of DyP, its activity for 1,4-diamino-2-sodium anthraquinone sulfonate decreases sharply when the H2O2 concentration exceeds 0.1 mM | Geotrichum candidum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
H2O2 | using Reactive Blue 5 as cosubstrate, at pH 3.2 and 30°C | Geotrichum candidum | |
0.036 | - |
H2O2 | using 1-amino-4-methylamino-2-sodium anthraquinone sulfonate as cosubstrate, at pH 3.2 and 30°C | Geotrichum candidum | |
0.054 | - |
Reactive Blue 5 | at pH 3.2 and 30°C | Geotrichum candidum | |
0.084 | - |
1-amino-4-methylamino-2-sodium anthraquinone sulfonate | at pH 3.2 and 30°C | Geotrichum candidum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no enhancement of the DyP activity by the addition of Mn2+ is observed | Geotrichum candidum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
1 * 55000, gel filtration | Geotrichum candidum |
60000 | - |
SDS-PAGE | Geotrichum candidum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geotrichum candidum | - |
strain Dec 1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | DyP is glycosylated with N-acetylglucosamine and mannose (17% (w/w)) | Geotrichum candidum |
Purification (Comment) | Organism |
---|---|
Super Q 650M column chromatography and butyl Toyopearl column chromatography | Geotrichum candidum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.28 | - |
crude enzyme, in 25 mM citrate buffer (pH 3.2), at 30°C | Geotrichum candidum |
57 | - |
after 210fold purification, in 25 mM citrate buffer (pH 3.2), at 30°C | Geotrichum candidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-diamino-2-sodium anthraquinone sulfonate + H2O2 + H+ | - |
Geotrichum candidum | ? | - |
? | |
1-amino-4-(3-amino-4-sodium-sulfonoanilino)-2-sodium anthraquinone sulfonate + H2O2 + H+ | - |
Geotrichum candidum | ? | - |
? | |
1-amino-4-methylamino-2-sodium anthraquinone sulfonate + H2O2 + H+ | - |
Geotrichum candidum | ? | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | - |
Geotrichum candidum | ? | - |
? | |
guaiacol + H2O2 + H+ | - |
Geotrichum candidum | ? | - |
? | |
additional information | higher decolorizing activity is observed for anthraquinone dyes than for azo dyes. DyP does not oxidize veratryl alcohol | Geotrichum candidum | ? | - |
? | |
Reactive Black 5 + H2O2 + H+ | - |
Geotrichum candidum | oxidized Reactive Black 5 + H2O | - |
? | |
Reactive Blue 114 + H2O2 + H+ | - |
Geotrichum candidum | oxidized Reactive Blue 114 + H2O | - |
? | |
Reactive Blue 182 + H2O2 + H+ | - |
Geotrichum candidum | oxidized Reactive Blue 182 + H2O | - |
? | |
Reactive Blue 19 + H2O2 + H+ | - |
Geotrichum candidum | oxidized Reactive Blue 19 + H2O | - |
? | |
Reactive Blue 5 + H2O2 + H+ | - |
Geotrichum candidum | oxidized Reactive Blue 5 + H2O | - |
? | |
Reactive Red 33 + H2O2 + H+ | - |
Geotrichum candidum | oxidized Reactive Red 33 + H2O | - |
? | |
Reactive Yellow 2 + H2O2 + H+ | - |
Geotrichum candidum | oxidized Reactive Yellow 2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, gel filtration | Geotrichum candidum |
Synonyms | Comment | Organism |
---|---|---|
DyP | - |
Geotrichum candidum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Geotrichum candidum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 35 | - |
Geotrichum candidum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | DyP activity is restored at 30°C after treatment at 40 and 50°C for 11 h, DyP activity is stable after incubation at 50°C for 11 h. When DyP is incubated at 30 and 40°C for 14 days, the inactivation rates are 37 and 59%, respectively. When DyP is heated at 60°C for 3 h, 35% of the initial activity is lost | Geotrichum candidum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
260 | - |
H2O2 | at pH 3.2 and 30°C | Geotrichum candidum | |
260 | - |
Reactive Blue 5 | at pH 3.2 and 30°C | Geotrichum candidum | |
270 | - |
1-amino-4-methylamino-2-sodium anthraquinone sulfonate | at pH 3.2 and 30°C | Geotrichum candidum | |
270 | - |
H2O2 | using AQ2 as cosubstrate, at pH 3.2 and 30°C | Geotrichum candidum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
using 1-amino-4-methylamino-2-sodium anthraquinone sulfonate as substrate | Geotrichum candidum |
3.2 | - |
using Reactive Blue 5, Reactive Blue 19, 1-amino-4-(3-amino-4-sodium-sulfonoanilino)-2-sodium anthraquinone sulfonate, 1,4-diamino-2-sodium anthraquinone sulfonate, Reactive Black 5, Reactive Red 33, or Reactive Yellow 2 as substrate | Geotrichum candidum |
4 | - |
using Reactive Blue 182 or Reactive Blue 114 as substrate | Geotrichum candidum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Geotrichum candidum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Geotrichum candidum | isoelectric focusing | - |
3.8 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3200 | - |
1-amino-4-methylamino-2-sodium anthraquinone sulfonate | at pH 3.2 and 30°C | Geotrichum candidum | |
4800 | - |
Reactive Blue 5 | at pH 3.2 and 30°C | Geotrichum candidum | |
7600 | - |
H2O2 | using AQ2 as cosubstrate, at pH 3.2 and 30°C | Geotrichum candidum | |
10000 | - |
H2O2 | using Reactive Blue 5 as cosubstrate, at pH 3.2 and 30°C | Geotrichum candidum |